Unfortunately, false negative test results are often reported. Using these Ct cutoff values, false negative results could be reliably identified. RPP30 could serve as an indicator for cellular content, or a surrogate indicator for specimen quality. At present, viral nucleic acids are generally sampled using throat swabs and detected by quantitative real-time reverse transcription polymerase chain reaction qRT-PCR. However, the capacity for SARS-CoV-2 nucleic acid detection is largely limited by the number of instruments, kits, and experienced laboratory personnel available. These limitations have caused low screening efficiency, which leads to a lag in identifying potential infections and may then exacerbate the spread of COVID Carter et al.
Wenzhou is located at the extreme south east of Zhejiang Province with its borders connecting to Lishui on the west, Taizhou on the north, and Fujian to the south. It is surrounded by mountains, the East China Sea , and islands, while its lowlands are almost entirely along its East China Sea coast, which is nearly kilometres miles in length. It is situated in a mountainous region and, as a result, has been isolated for most of its history from the rest of the country, making the local culture and language very distinct not only from the rest of China but from neighbouring areas as well. The city is also the native land of many emigrants to Europe and the United States , with many of these Wenzhounese emigrants being entrepreneurs who start restaurants, retail and wholesale businesses in their adopted countries. After the victory, emperor Hui of Han , the second emperor of Han dynasty named Zhou Yao the King of Dong'ou Wenzhou , and under the administration of emperor Hui, Wenzhou became the capital of the Dong'ou Kingdom which is the modern-day area of southern Zhejiang.